Hotspot

ycf3-trnS-GGA

LSC · 44030-44603 · 574 bp

Back to Dracaena

Pi 0.0287
MarkerSeek score 68.60
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
574
variable_sites
86
pi
0.0287
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 758-759 bp 1.0 74.3
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 759-760 bp 1.0 74.3
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTGAA 45.0 45.0 56.4 57.8 707-708 bp 1.0 74.0
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA TTCCAATGCTACGCCTTGAA 45.0 45.0 56.4 57.8 708-709 bp 1.0 74.0
ycf3-trnS-GGA_p5 TCTCCTGAAGTTGTCGGAAT ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.2 58.2 757-758 bp 1.0 72.5