Hotspot

psbM-trnD-GUC

LSC · 29890-30609 · 720 bp

Back to Dracaena

Pi 0.0108

MarkerSeek score 62.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 37
pi: 0.0108
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TGCTATACACCTTGCTGGGA	45.0	50.0	55.8	58.4	853-868 bp	1.0	63.1
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	827-842 bp	1.0	62.8
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	ACACCTTGCTGGGATTGTAG	45.0	50.0	55.8	57.5	847-862 bp	1.0	62.5
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	CACCTTGCTGGGATTGTAGT	45.0	50.0	55.8	57.5	846-861 bp	1.0	62.5
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	ATGCTATACACCTTGCTGGG	45.0	50.0	55.8	57.4	854-869 bp	1.0	61.3