Hotspot

trnS-GCU-trnG-UCC

LSC · 7958-9039 · 1082 bp

Back to Dracaena

Pi 0.0222
MarkerSeek score 66.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1082
variable_sites
69
pi
0.0222
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 AATCTGCCGCTTTAGTCCAC CGAACGAATCACACTTTTACCA 50.0 40.9 58.3 57.3 1113-1305 bp 1.0 65.0
trnS-GCU-trnG-UCC_p2 CAATCTGCCGCTTTAGTCCA CGAACGAATCACACTTTTACCA 50.0 40.9 58.3 57.3 1114-1306 bp 1.0 64.9
trnS-GCU-trnG-UCC_p3 GCTTTAGTCCACTCAGCCAT CGAACGAATCACACTTTTACCA 50.0 40.9 57.6 57.3 1105-1297 bp 1.0 63.7
trnS-GCU-trnG-UCC_p4 AGTCCACTCAGCCATCTCTC CGAACGAATCACACTTTTACCA 55.0 40.9 58.5 57.3 1100-1292 bp 1.0 62.9
trnS-GCU-trnG-UCC_p5 AATCTGCCGCTTTAGTCCAC TCGAACGAATCACACTTTTACC 50.0 40.9 58.3 57.0 1114-1306 bp 1.0 62.9