Hotspot

trnQ-UUG-psbK

LSC · 6695-7007 · 313 bp

Back to Dracaena

Pi 0.0494

MarkerSeek score 72.80

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 58
pi: 0.0494
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	AACAAAGAAGGGGTTGGGTC	AGCAGCTTGCCAAACAAAAG	50.0	45.0	57.9	58.1	659-660 bp	1.0	88.0
trnQ-UUG-psbK_p2	GAACAAAGAAGGGGTTGGGT	AGCAGCTTGCCAAACAAAAG	50.0	45.0	57.9	58.1	660-661 bp	1.0	88.0
trnQ-UUG-psbK_p3	AACAAAGAAGGGGTTGGGTC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	57.9	57.9	664-665 bp	1.0	87.9
trnQ-UUG-psbK_p4	GAACAAAGAAGGGGTTGGGT	CTTACAGCAGCTTGCCAAAC	50.0	50.0	57.9	57.9	665-666 bp	1.0	87.9
trnQ-UUG-psbK_p5	AACAAAGAAGGGGTTGGGTC	CTTCACGTCCAGGATTACGG	50.0	55.0	57.9	58.1	1209-1241 bp	1.0	86.3