Hotspot

ycf1

SSC · 122116-127458 · 5343 bp

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Pi 0.0138
MarkerSeek score 51.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5343
variable_sites
217
pi
0.0138
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CGCGGTTTGTTACAACGATT ATGGAAACTGAACTGCCGTT 45.0 45.0 57.7 58.0 482 bp 0.888889 77.5
ycf1_p2 CGCGGTTTGTTACAACGATT CGTTCCCAAAGGCGTAAAAC 45.0 50.0 57.7 58.0 1088-1098 bp 0.888889 77.3
ycf1_p3 CGCGGTTTGTTACAACGATT GAAACTGAACTGCCGTTTGG 45.0 50.0 57.7 57.9 479 bp 0.888889 76.7
ycf1_p4 CGCGGTTTGTTACAACGATT GGAAACTGAACTGCCGTTTG 45.0 50.0 57.7 57.9 480 bp 0.888889 76.7
ycf1_p5 CGCGGTTTGTTACAACGATT AATGGAAACTGAACTGCCGT 45.0 45.0 57.7 58.0 483 bp 0.666667 67.2