Hotspot

rpl16

LSC · 79324-80852 · 1529 bp

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Pi 0.0142
MarkerSeek score 56.00
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1529
variable_sites
59
pi
0.0142
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 ATCGGCGATTACTAGCTCCT GTATGGAGGGAATTGCACGT 50.0 50.0 58.1 58.0 2398-2439 bp 1.0 83.0
rpl16_p2 ATCGGCGATTACTAGCTCCT TGCACGTGTTGAATGGATCA 50.0 45.0 58.1 58.1 2046-2087 bp 1.0 82.7
rpl16_p3 ATCGTCCCGTTGTTACGTTT GTATGGAGGGAATTGCACGT 45.0 50.0 57.8 58.0 2531-2572 bp 1.0 82.4
rpl16_p4 ATCGTCCCGTTGTTACGTTT TGCACGTGTTGAATGGATCA 45.0 45.0 57.8 58.1 2179-2220 bp 1.0 82.1
rpl16_p5 ATCGGCGATTACTAGCTCCT GAGGGAATTGCACGTATCGA 50.0 50.0 58.1 57.8 2393-2434 bp 1.0 81.6