Hotspot

rpl32-trnL-CUA

SSC · 111949-112402 · 454 bp

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Pi 0.0369
MarkerSeek score 74.80
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
454
variable_sites
43
pi
0.0369
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 TTAAATGGGCGCTCTCGTAC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 888-1316 bp 0.777778 76.0
rpl32-trnL-CUA_p2 TTAAATGGGCGCTCTCGTAC TAACCACTGGGACTGGAGAG 50.0 55.0 58.1 58.1 1460-1889 bp 0.777778 75.1
rpl32-trnL-CUA_p3 TTAAATGGGCGCTCTCGTAC AACAAAGCGCCATAGCCTAA 50.0 45.0 58.1 57.9 1504-1933 bp 0.777778 74.5
rpl32-trnL-CUA_p4 AGATTAAATGGGCGCTCTCG TAACCACTGGGACTGGAGAG 50.0 55.0 58.1 58.1 1463 bp 0.111111 42.9
rpl32-trnL-CUA_p5 AGATTAAATGGGCGCTCTCG AACAAAGCGCCATAGCCTAA 50.0 45.0 58.1 57.9 1507 bp 0.111111 42.4