Hotspot

petA-psbJ

LSC · 61088-61943 · 856 bp

Back to Draba

Pi 0.0237
MarkerSeek score 68.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
856
variable_sites
58
pi
0.0237
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TAGCATCGGTTGTTTTGGCA CAGGATTAGGTTCATCCCTGT 45.0 47.6 58.4 56.8 965-1074 bp 1.0 67.6
petA-psbJ_p2 TAGCATCGGTTGTTTTGGCA TCAGGATTAGGTTCATCCCTGT 45.0 45.5 58.4 58.3 966-1075 bp 1.0 67.2
petA-psbJ_p3 TAGCATCGGTTGTTTTGGCA TCAGGATTAGGTTCATCCCTG 45.0 47.6 58.4 56.5 966-1075 bp 1.0 65.3
petA-psbJ_p4 TTAGCATCGGTTGTTTTGGC CAGGATTAGGTTCATCCCTGT 45.0 47.6 57.3 56.8 966-1075 bp 1.0 65.0
petA-psbJ_p5 TTAGCATCGGTTGTTTTGGC TCAGGATTAGGTTCATCCCTGT 45.0 45.5 57.3 58.3 967-1076 bp 1.0 64.6