Hotspot

trnM-AUG-atpE

LSC · 50669-50796 · 128 bp

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Pi 0.0309
MarkerSeek score 64.10
Primer available yes
Label trnM-AUG-atpE

Feature Metadata

label_name
trnM-AUG-atpE
feature_type
igs
region
LSC
length_bp
128
variable_sites
10
pi
0.0309
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-AUG-atpE_p1 GGCAGGAGTCATTGGTTCAA ACGAGTCGAGGCTCTCAATA 50.0 50.0 58.1 58.0 181-194 bp 1.0 87.2
trnM-AUG-atpE_p2 GGCAGGAGTCATTGGTTCAA AGTCGAGGCTCTCAATACGA 50.0 50.0 58.1 58.0 178-191 bp 1.0 87.2
trnM-AUG-atpE_p3 TTGCTTTCATACGGCAGGAG AGTCGAGGCTCTCAATACGA 50.0 50.0 58.3 58.0 190-203 bp 1.0 85.6
trnM-AUG-atpE_p4 TTGCTTTCATACGGCAGGAG ACGAGTCGAGGCTCTCAATA 50.0 50.0 58.3 58.0 193-206 bp 1.0 85.5
trnM-AUG-atpE_p5 CATACGGCAGGAGTCATTGG AGTCGAGGCTCTCAATACGA 55.0 50.0 58.4 58.0 183-196 bp 1.0 84.5