Hotspot

ndhC-trnV-GUA

LSC · 48896-49728 · 833 bp

Back to Draba

Pi 0.0176
MarkerSeek score 67.70
Primer available yes
Label ndhC-trnV-GUA

Feature Metadata

label_name
ndhC-trnV-GUA
feature_type
igs
region
LSC
length_bp
833
variable_sites
40
pi
0.0176
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-GUA_p1 TGCCAAAACAGGAATAGCACT GTTCGAGTCCGTATAGCCCT 42.9 55.0 58.1 58.7 875-929 bp 1.0 73.0
ndhC-trnV-GUA_p2 TGCCAAAACAGGAATAGCACT TTCGAGTCCGTATAGCCCTA 42.9 50.0 58.1 57.1 874-928 bp 1.0 71.0
ndhC-trnV-GUA_p3 TGCCAAAACAGGAATAGCAC GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.6 58.7 875-929 bp 1.0 70.9
ndhC-trnV-GUA_p4 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCAG 42.9 47.6 58.1 58.1 903-957 bp 1.0 69.9
ndhC-trnV-GUA_p5 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCA 42.9 45.0 58.1 56.9 903-957 bp 1.0 69.6