Hotspot

trnE-GAA-trnT-ACC

LSC · 29617-30307 · 691 bp

Back to Draba

Pi 0.0267
MarkerSeek score 70.70
Primer available yes
Label trnE-GAA-trnT-ACC

Feature Metadata

label_name
trnE-GAA-trnT-ACC
feature_type
igs
region
LSC
length_bp
691
variable_sites
56
pi
0.0267
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-GAA-trnT-ACC_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 830-845 bp 1.0 85.0
trnE-GAA-trnT-ACC_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 809-824 bp 1.0 84.7
trnE-GAA-trnT-ACC_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 806-821 bp 1.0 84.5
trnE-GAA-trnT-ACC_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 792-807 bp 1.0 84.5
trnE-GAA-trnT-ACC_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 771-786 bp 1.0 84.5