Hotspot

psbM-trnD-GAC

LSC · 27867-28904 · 1038 bp

Back to Draba

Pi 0.0231
MarkerSeek score 69.50
Primer available yes
Label psbM-trnD-GAC

Feature Metadata

label_name
psbM-trnD-GAC
feature_type
igs
region
LSC
length_bp
1038
variable_sites
67
pi
0.0231
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GAC_p1 AGGAACGAGAATGAAGAGTGC GTTCGAGCCCCGTCAGTC 47.6 66.7 58.0 60.1 1109-1128 bp 1.0 47.0
psbM-trnD-GAC_p2 ACGAGAATGAAGAGTGCAGT GTTCGAGCCCCGTCAGTC 45.0 66.7 56.9 60.1 1105-1124 bp 1.0 47.0
psbM-trnD-GAC_p3 GGAACGAGAATGAAGAGTGCA GTTCGAGCCCCGTCAGTC 47.6 66.7 58.3 60.1 1108-1127 bp 1.0 47.0
psbM-trnD-GAC_p4 AACGAGAATGAAGAGTGCAGT GTTCGAGCCCCGTCAGTC 42.9 66.7 57.6 60.1 1106-1125 bp 1.0 47.0
psbM-trnD-GAC_p5 GGAACGAGAATGAAGAGTGC GTTCGAGCCCCGTCAGTC 50.0 66.7 56.5 60.1 1108-1127 bp 1.0 47.0