Hotspot

atpH-atpI

LSC · 12700-13144 · 445 bp

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Pi 0.0234
MarkerSeek score 76.10
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
445
variable_sites
25
pi
0.0234
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 GTCCAATAGAAGCAAGCCCA TACAAGCGGGATTCAAGCTC 50.0 50.0 57.9 58.0 594-627 bp 1.0 84.0
atpH-atpI_p2 TACCTTGACCAACTCCAGGT TACAAGCGGGATTCAAGCTC 50.0 50.0 57.9 58.0 612-645 bp 1.0 83.9
atpH-atpI_p3 GCTGTACCTTGACCAACTCC TACAAGCGGGATTCAAGCTC 55.0 50.0 58.2 58.0 616-649 bp 1.0 83.3
atpH-atpI_p4 TACCTTGACCAACTCCAGGT TTTTTGCCACTTTAGCTGCG 50.0 45.0 57.9 57.9 589-622 bp 1.0 83.3
atpH-atpI_p5 GTCCAATAGAAGCAAGCCCA TTTTTGCCACTTTAGCTGCG 50.0 45.0 57.9 57.9 571-604 bp 1.0 83.3