Hotspot

psbK-psbI

LSC · 6452-6893 · 442 bp

Back to Draba

Pi 0.0258
MarkerSeek score 68.40
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
442
variable_sites
28
pi
0.0258
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCAGCTGTAAG ACAAAAAGTTTGAGAGTAAGCA 50.0 31.8 57.9 54.4 475-495 bp 1.0 48.7
psbK-psbI_p2 TGGCAAGCAGCTGTAAGTTT ACAAAAAGTTTGAGAGTAAGCA 45.0 31.8 58.3 54.4 472-492 bp 1.0 48.7
psbK-psbI_p3 TTGGCAAGCAGCTGTAAGTT ACAAAAAGTTTGAGAGTAAGCA 45.0 31.8 58.3 54.4 473-493 bp 1.0 48.7
psbK-psbI_p4 TTTGGCAAGCAGCTGTAAGT ACAAAAAGTTTGAGAGTAAGCA 45.0 31.8 58.3 54.4 474-494 bp 1.0 48.7
psbK-psbI_p5 GTTTGGCAAGCAGCTGTAAG ACAAAAAGTTTGAGAGTAAGCAT 50.0 30.4 57.9 55.0 475-495 bp 1.0 48.7