Hotspot

trnK-AAA-trnQ-CAA

LSC · 4376-5837 · 1462 bp

Back to Draba

Pi 0.0216
MarkerSeek score 73.00
Primer available yes
Label trnK-AAA-trnQ-CAA

Feature Metadata

label_name
trnK-AAA-trnQ-CAA
feature_type
igs
region
LSC
length_bp
1462
variable_sites
103
pi
0.0216
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-AAA-trnQ-CAA_p1 AAAGCCGAGTACTCTACCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 977-1564 bp 1.0 87.6
trnK-AAA-trnQ-CAA_p2 AAGCCGAGTACTCTACCGTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 976-1563 bp 1.0 87.6
trnK-AAA-trnQ-CAA_p3 TACCGTTGAGTTAGCAACCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 963-1550 bp 1.0 85.3
trnK-AAA-trnQ-CAA_p4 AGTCGCACTTAAAAGCCGAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.6 58.1 988-1575 bp 1.0 84.3
trnK-AAA-trnQ-CAA_p5 TCGCACTTAAAAGCCGAGTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 986-1573 bp 1.0 83.1