Hotspot

ycf1

SSC · 98146-105069 · 6924 bp

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Pi 0.188
MarkerSeek score 75.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
6924
variable_sites
1266
pi
0.188
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CAGCGCTTCCTAGTTTAGCA TTGCTCTGTCCGATTAAGCC 50.0 50.0 58.0 58.0 881 bp 0.5 59.7
ycf1_p2 ACAGCGCTTCCTAGTTTAGC TTGCTCTGTCCGATTAAGCC 50.0 50.0 58.0 58.0 882 bp 0.5 59.7
ycf1_p3 CAGCGCTTCCTAGTTTAGCA CCGATTAAGCCTTCTTCCCC 50.0 55.0 58.0 58.0 872 bp 0.5 59.7
ycf1_p4 CAGCGCTTCCTAGTTTAGCA CGATTAAGCCTTCTTCCCCC 50.0 55.0 58.0 58.0 871 bp 0.5 59.7
ycf1_p5 ACAGCGCTTCCTAGTTTAGC CGATTAAGCCTTCTTCCCCC 50.0 55.0 58.0 58.0 872 bp 0.5 59.7