Hotspot

ORF441

SSC · 113666-114991 · 1326 bp

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Pi 0.321

MarkerSeek score 83.40

Primer available yes

Label ORF441

Feature Metadata

label_name: ORF441
feature_type: gene
region: SSC
length_bp: 1326
variable_sites: 406
pi: 0.321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF441_p1	ACTGCCGAGGAATATCCCTT	ACCACTGAGCTACTGAGGAA	50.0	50.0	58.2	57.7	2008-2502 bp	1.0	88.0
ORF441_p2	ACTGCCGAGGAATATCCCTT	CCTTCGGCCTTTTCGTTTTG	50.0	50.0	58.2	58.2	2625 bp	0.5	56.6
ORF441_p3	ACTGCCGAGGAATATCCCTT	GAGGAACCTCAAAGTCGCTC	50.0	55.0	58.2	58.3	2488 bp	0.5	56.2
ORF441_p4	ACTGCCGAGGAATATCCCTT	TGGAAAAGGCCATTTCAGCA	50.0	45.0	58.2	58.3	1956 bp	0.5	56.2
ORF441_p5	TCGCATTTTTGATTTGCCGG	CCTTCGGCCTTTTCGTTTTG	45.0	50.0	58.3	58.2	2677 bp	0.5	55.8