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ORF342

LSC · 31254-32282 · 1029 bp

Back to Downingia

Pi 0.463

MarkerSeek score 80.30

Primer available yes

Label ORF342

Feature Metadata

label_name: ORF342
feature_type: gene
region: LSC
length_bp: 1029
variable_sites: 397
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF342_p1	ACAGCCGTACCATTAACGTC	ACTTCGTCCCCAGACTACAA	50.0	50.0	58.0	58.0	1780 bp	0.5	60.0
ORF342_p2	ACAGCCGTACCATTAACGTC	TTCGTCCCCAGACTACAAGT	50.0	50.0	58.0	58.0	1778 bp	0.5	60.0
ORF342_p3	CACGAACAGCCGTACCATTA	ACTTCGTCCCCAGACTACAA	50.0	50.0	58.0	58.0	1785 bp	0.5	59.9
ORF342_p4	CACGAACAGCCGTACCATTA	TTCGTCCCCAGACTACAAGT	50.0	50.0	58.0	58.0	1783 bp	0.5	59.9
ORF342_p5	ACAGCCGTACCATTAACGTC	GGTGCAGAGTCAAAAAGGGA	50.0	50.0	58.0	58.0	1630 bp	0.5	59.8