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ORF213

LSC · 11188-11829 · 642 bp

Back to Downingia

Pi 0.269

MarkerSeek score 71.50

Primer available yes

Label ORF213

Feature Metadata

label_name: ORF213
feature_type: gene
region: LSC
length_bp: 642
variable_sites: 73
pi: 0.269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.42
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF213_p1	TTCGGGAGCGAACATCAATT	ACCGGCCCAATCAAAAATCT	45.0	45.0	57.9	58.1	980-1409 bp	1.0	90.6
ORF213_p2	ATTCTTGCAAGGGATAGCCG	TGGGCGCTAATTGCAATTTG	50.0	45.0	58.0	58.0	1713 bp	0.5	59.7
ORF213_p3	ATTCTTGCAAGGGATAGCCG	ACCGGCCCAATCAAAAATCT	50.0	45.0	58.0	58.1	1625 bp	0.5	59.3
ORF213_p4	TTCGGGAGCGAACATCAATT	TGGGCGCTAATTGCAATTTG	45.0	45.0	57.9	58.0	1497 bp	0.5	59.1
ORF213_p5	TTTTCGAAGCTCGCTTCCTT	TGGGCGCTAATTGCAATTTG	45.0	45.0	58.1	58.0	1520 bp	0.5	59.0