Hotspot

ORF175

IRa · 134260-134787 · 528 bp

Back to Downingia

Pi 0.452

MarkerSeek score 75.80

Primer available yes

Label ORF175

Feature Metadata

label_name: ORF175
feature_type: gene
region: IRa
length_bp: 528
variable_sites: 173
pi: 0.452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF175_p1	CTATCGAGTGCAGAGAGGGA	TCACTGGACTGTCTACGGTT	55.0	50.0	58.0	58.0	1038 bp	0.5	59.7
ORF175_p2	CTATCGAGTGCAGAGAGGGA	TTCACTGGACTGTCTACGGT	55.0	50.0	58.0	58.0	1039 bp	0.5	59.7
ORF175_p3	TCTATCGAGTGCAGAGAGGG	TCACTGGACTGTCTACGGTT	55.0	50.0	58.0	58.0	1039 bp	0.5	59.7
ORF175_p4	TCTATCGAGTGCAGAGAGGG	TTCACTGGACTGTCTACGGT	55.0	50.0	58.0	58.0	1040 bp	0.5	59.7
ORF175_p5	CTATCGAGTGCAGAGAGGGA	TTTGCATTCCGGCGAAAATC	55.0	45.0	58.0	58.0	1180 bp	0.5	59.7