Hotspot

cemA-pafII

LSC · 59248-60088 · 841 bp

Back to Downingia

Pi 0.113

MarkerSeek score 81.20

Primer available yes

Label cemA-pafII

Feature Metadata

label_name: cemA-pafII
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 93
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-pafII_p1	CGTCCAAGAGGAGAAGTTCG	TCTTTATGCTCAGCGTGTCC	55.0	50.0	58.0	58.0	1202-1203 bp	1.0	88.7
cemA-pafII_p2	CGTCCAAGAGGAGAAGTTCG	CTTTATGCTCAGCGTGTCCT	55.0	50.0	58.0	58.0	1201-1202 bp	1.0	88.7
cemA-pafII_p3	CGTCCAAGAGGAGAAGTTCG	GGGATCGCGGGTCTTTTTAT	55.0	50.0	58.0	58.0	1399-1400 bp	1.0	87.9
cemA-pafII_p4	CGTCCAAGAGGAGAAGTTCG	AAATGGAAACCGGAGCAAGT	55.0	45.0	58.0	58.0	1627 bp	0.5	59.8
cemA-pafII_p5	CGTCCAAGAGGAGAAGTTCG	ATTTCGTGGAATGTCGGGAG	55.0	50.0	58.0	58.0	1358 bp	0.5	59.7