Hotspot

trnT (UGU)-trnL (UAA)

LSC · 49763-50427 · 665 bp

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Pi 0.1
MarkerSeek score 81.80
Primer available yes
Label trnT (UGU)-trnL (UAA)

Feature Metadata

label_name
trnT (UGU)-trnL (UAA)
feature_type
igs
region
LSC
length_bp
665
variable_sites
65
pi
0.1
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT (UGU)-trnL (UAA)_p1 CTCTTGGGCTTTGGGACTTT ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1186 bp 0.5 60.0
trnT (UGU)-trnL (UAA)_p2 CTCTTGGGCTTTGGGACTTT CACGCTTTCGGAAGTTTCAC 50.0 50.0 58.0 58.0 1438 bp 0.5 59.8
trnT (UGU)-trnL (UAA)_p3 TATCCTCTTGGGCTTTGGGA ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1190 bp 0.5 59.8
trnT (UGU)-trnL (UAA)_p4 TATCCTCTTGGGCTTTGGGA CACGCTTTCGGAAGTTTCAC 50.0 50.0 58.0 58.0 1442 bp 0.5 59.7
trnT (UGU)-trnL (UAA)_p5 CTCTTGGGCTTTGGGACTTT TCACGCTTTCGGAAGTTTCA 50.0 45.0 58.0 58.1 1439 bp 0.5 59.5