Hotspot

pafI-trnS (GGA)

LSC · 47585-48480 · 896 bp

Back to Downingia

Pi 0.122
MarkerSeek score 81.00
Primer available yes
Label pafI-trnS (GGA)

Feature Metadata

label_name
pafI-trnS (GGA)
feature_type
igs
region
LSC
length_bp
896
variable_sites
107
pi
0.122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI-trnS (GGA)_p1 GCCTAACCATTCTTCGTCGT AGGTACGTTGGGTGAAGGTA 50.0 50.0 58.0 58.0 1473 bp 0.5 59.8
pafI-trnS (GGA)_p2 GCCTAACCATTCTTCGTCGT TAGGTACGTTGGGTGAAGGT 50.0 50.0 58.0 58.0 1474 bp 0.5 59.8
pafI-trnS (GGA)_p3 TTTTTCCCCTGGAGTTGTCG AGGTACGTTGGGTGAAGGTA 50.0 50.0 58.0 58.0 1102 bp 0.5 59.7
pafI-trnS (GGA)_p4 TTTTTCCCCTGGAGTTGTCG TAGGTACGTTGGGTGAAGGT 50.0 50.0 58.0 58.0 1103 bp 0.5 59.7
pafI-trnS (GGA)_p5 GCCTAACCATTCTTCGTCGT GTTATCGCTGCAAACCCCTA 50.0 50.0 58.0 58.0 1855 bp 0.5 59.7