Hotspot

trnK (UUU)-rps16

LSC · 10373-10883 · 511 bp

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Pi 0.124
MarkerSeek score 81.50
Primer available yes
Label trnK (UUU)-rps16

Feature Metadata

label_name
trnK (UUU)-rps16
feature_type
igs
region
LSC
length_bp
511
variable_sites
61
pi
0.124
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK (UUU)-rps16_p1 ATCCATTGGACCCAACCCTA ACGGTAACTATTCGTGTGCC 50.0 50.0 58.0 58.0 1336 bp 0.5 60.0
trnK (UUU)-rps16_p2 TATCCATTGGACCCAACCCT ACGGTAACTATTCGTGTGCC 50.0 50.0 58.0 58.0 1337 bp 0.5 60.0
trnK (UUU)-rps16_p3 CCTTACCCGTATTCAGGCAC ACGGTAACTATTCGTGTGCC 55.0 50.0 58.1 58.0 1478 bp 0.5 59.6
trnK (UUU)-rps16_p4 ATCCATTGGACCCAACCCTA CTCTCACCGAAATCAGGAGC 50.0 55.0 58.0 58.1 1105 bp 0.5 59.4
trnK (UUU)-rps16_p5 TATCCATTGGACCCAACCCT CTCTCACCGAAATCAGGAGC 50.0 55.0 58.0 58.1 1106 bp 0.5 59.4