Hotspot

psbA-rbcL

LSC · 1477-2299 · 823 bp

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Pi 0.112
MarkerSeek score 82.00
Primer available yes
Label psbA-rbcL

Feature Metadata

label_name
psbA-rbcL
feature_type
igs
region
LSC
length_bp
823
variable_sites
91
pi
0.112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-rbcL_p1 CGTCTCTCTAAAATTGCAGTCA ATTTGAGTTTGCCGCAATGG 40.9 45.0 56.5 57.9 877-908 bp 1.0 60.4
psbA-rbcL_p2 CGTCTCTCTAAAATTGCAGTCA TGAGTTTGCCGCAATGGATA 40.9 45.0 56.5 57.9 874-905 bp 1.0 60.1
psbA-rbcL_p3 CGTCTCTCTAAAATTGCAGTCA AGTTTGCCGCAATGGATACT 40.9 45.0 56.5 57.9 872-903 bp 1.0 60.0
psbA-rbcL_p4 CGTCTCTCTAAAATTGCAGTCA GAGTTTGCCGCAATGGATAC 40.9 50.0 56.5 57.5 873-904 bp 1.0 57.2
psbA-rbcL_p5 CGTCTCTCTAAAATTGCAGTCAT ATTTGAGTTTGCCGCAATGG 39.1 45.0 57.0 57.9 877-908 bp 1.0 56.2