Hotspot

ycf1

SSC · 123766-129138 · 5373 bp

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Pi 0.0251

MarkerSeek score 54.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5373
variable_sites: 201
pi: 0.0251
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCGCGAATACCTTGTGAGAA	TGTAACAAACCGCGTTATGG	50.0	45.0	58.0	56.7	1527 bp	1.0	74.3
ycf1_p2	CCGCGAATACCTTGTGAGAA	GTAACAAACCGCGTTATGGT	50.0	45.0	58.0	56.7	1526 bp	1.0	74.3
ycf1_p3	ACCAATCCAGGGCTCCATAT	TGTAACAAACCGCGTTATGG	50.0	45.0	58.2	56.7	981 bp	0.666667	58.0
ycf1_p4	TCTTGGTGTGATAGAGCCGA	TGTAACAAACCGCGTTATGG	50.0	45.0	58.2	56.7	1412 bp	0.333333	42.1
ycf1_p5	TCTTGGTGTGATAGAGCCGA	GTAACAAACCGCGTTATGGT	50.0	45.0	58.2	56.7	1411 bp	0.333333	42.1