Hotspot

rpl16

LSC · 80533-82093 · 1561 bp

Back to Dontostemon

Pi 0.0321

MarkerSeek score 69.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1561
variable_sites: 68
pi: 0.0321
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	TGCACGTGTTGAATGGATCA	50.0	45.0	58.0	58.1	2022-2036 bp	1.0	85.2
rpl16_p2	ACACATCAATTCTCGAGCCC	GTATGGAGGGAATTGCACGT	50.0	50.0	58.0	58.0	2374-2388 bp	1.0	85.0
rpl16_p3	ATCGGCGATTACTAGCTCCT	TGCACGTGTTGAATGGATCA	50.0	45.0	58.1	58.1	2053-2067 bp	1.0	84.7
rpl16_p4	ATCGGCGATTACTAGCTCCT	GTATGGAGGGAATTGCACGT	50.0	50.0	58.1	58.0	2405-2419 bp	1.0	84.5
rpl16_p5	ACACATCAATTCTCGAGCCC	GAGGGAATTGCACGTATCGA	50.0	50.0	58.0	57.8	2369-2383 bp	1.0	83.5