Hotspot

rpl32-trnL-CUA

SSC · 113290-114136 · 847 bp

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Pi 0.0921
MarkerSeek score 87.50
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
847
variable_sites
110
pi
0.0921
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 TGGACATCGTTGAAAGCTTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 55.9 57.9 967-992 bp 1.0 74.0
rpl32-trnL-CUA_p2 TTGGACATCGTTGAAAGCTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 55.9 57.9 968-993 bp 1.0 74.0
rpl32-trnL-CUA_p3 TGGACATCGTTGAAAGCTTT AGCAGCGTGTCTACCAATTT 40.0 45.0 55.9 57.8 960-985 bp 1.0 73.5
rpl32-trnL-CUA_p4 TTGGACATCGTTGAAAGCTT AGCAGCGTGTCTACCAATTT 40.0 45.0 55.9 57.8 961-986 bp 1.0 73.5
rpl32-trnL-CUA_p5 TGGACATCGTTGAAAGCTTT CTAAGAGCAGCGTGTCTACC 40.0 55.0 55.9 57.8 965-990 bp 1.0 73.5