Hotspot

trnP-CCA-psaJ

LSC · 65790-66152 · 363 bp

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Pi 0.0425

MarkerSeek score 74.40

Primer available yes

Label trnP-CCA-psaJ

Feature Metadata

label_name: trnP-CCA-psaJ
feature_type: igs
region: LSC
length_bp: 363
variable_sites: 22
pi: 0.0425
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-CCA-psaJ_p1	CCCGTGACATTTTGTACCCA	TGGCGCTACGGAAAGATATG	50.0	50.0	58.1	57.9	451-461 bp	1.0	83.5
trnP-CCA-psaJ_p2	CCCGTGACATTTTGTACCCA	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	450-460 bp	1.0	83.5
trnP-CCA-psaJ_p3	ACCCGTGACATTTTGTACCC	TGGCGCTACGGAAAGATATG	50.0	50.0	58.1	57.9	452-462 bp	1.0	83.4
trnP-CCA-psaJ_p4	ACCCGTGACATTTTGTACCC	GGCGCTACGGAAAGATATGT	50.0	50.0	58.1	57.8	451-461 bp	1.0	83.4
trnP-CCA-psaJ_p5	ACAGGATTTGAACCCGTGAC	TGGCGCTACGGAAAGATATG	50.0	50.0	58.1	57.9	463-473 bp	1.0	83.3