Hotspot

ycf4-cemA

LSC · 59636-60057 · 422 bp

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Pi 0.0483

MarkerSeek score 71.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 422
variable_sites: 30
pi: 0.0483
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AGGTCAAGGGGCTATTCCTT	GGCGAATGGAATCCGATACA	50.0	50.0	58.0	57.8	1037-1059 bp	1.0	81.6
ycf4-cemA_p2	TTGGCTTACTTCTTGCGTGT	GGCGAATGGAATCCGATACA	45.0	50.0	58.0	57.8	961-983 bp	1.0	81.3
ycf4-cemA_p3	AACGGGGTCTCGAAAAACAA	GGCGAATGGAATCCGATACA	45.0	50.0	58.0	57.8	1439-1461 bp	1.0	80.9
ycf4-cemA_p4	GTTGCTATTTGTGGTGCACC	GGCGAATGGAATCCGATACA	50.0	50.0	57.9	57.8	1248-1270 bp	1.0	80.8
ycf4-cemA_p5	CGTTGGGGATTTCCTGGAAA	GGCGAATGGAATCCGATACA	50.0	50.0	58.1	57.8	1162-1184 bp	1.0	80.8