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trnT-ACC-psbD

LSC · 30627-31868 · 1242 bp

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Pi 0.0301

MarkerSeek score 75.90

Primer available yes

Label trnT-ACC-psbD

Feature Metadata

label_name: trnT-ACC-psbD
feature_type: igs
region: LSC
length_bp: 1242
variable_sites: 52
pi: 0.0301
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACC-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1292-1396 bp	1.0	86.2
trnT-ACC-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1295-1399 bp	1.0	86.0
trnT-ACC-psbD_p3	CCAAGACGGCCCTTTTAACT	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1314-1418 bp	1.0	85.9
trnT-ACC-psbD_p4	ACGGCCCTTTTAACTCAGTG	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1309-1413 bp	1.0	85.8
trnT-ACC-psbD_p5	GACGGCCCTTTTAACTCAGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	57.8	58.0	1310-1414 bp	1.0	85.3