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atpH-atpI

LSC · 13306-13796 · 491 bp

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Pi 0.0279

MarkerSeek score 75.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 19
pi: 0.0279
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACCGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.1	58.0	586-608 bp	1.0	83.8
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	644-666 bp	1.0	83.3
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	626-648 bp	1.0	83.3
atpH-atpI_p4	AACCGAAGCAGCAGAAATCA	TTTTTGCCACTTTAGCTGCG	45.0	45.0	58.1	57.9	563-585 bp	1.0	83.1
atpH-atpI_p5	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	55.0	50.0	58.2	58.0	648-670 bp	1.0	82.6