Hotspot

trnR-AGA-atpA

LSC · 9401-9715 · 315 bp

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Pi 0.0472
MarkerSeek score 72.20
Primer available yes
Label trnR-AGA-atpA

Feature Metadata

label_name
trnR-AGA-atpA
feature_type
igs
region
LSC
length_bp
315
variable_sites
19
pi
0.0472
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-AGA-atpA_p1 AGCCTTCCAAGCTAACGATG AGGGCCGCTAAATTAAGCTC 50.0 50.0 58.0 58.0 1174-1212 bp 1.0 85.5
trnR-AGA-atpA_p2 AGCCTTCCAAGCTAACGATG GGGCCGCTAAATTAAGCTCT 50.0 50.0 58.0 58.0 1173-1211 bp 1.0 85.5
trnR-AGA-atpA_p3 ACCCTTAGCCTTCCAAGCTA AGGGCCGCTAAATTAAGCTC 50.0 50.0 58.0 58.0 1180-1218 bp 1.0 85.4
trnR-AGA-atpA_p4 ACCCTTAGCCTTCCAAGCTA GGGCCGCTAAATTAAGCTCT 50.0 50.0 58.0 58.0 1179-1217 bp 1.0 85.4
trnR-AGA-atpA_p5 CGACGTCGACTAAAACCCTT GGGCCGCTAAATTAAGCTCT 50.0 50.0 57.9 58.0 1193-1231 bp 1.0 85.2