Hotspot

psbK-psbI

LSC · 7063-7486 · 424 bp

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Pi 0.0489

MarkerSeek score 78.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 27
pi: 0.0489
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCAGCTGTAAG	TTCTTCACGTCCCGGATTAC	50.0	50.0	57.9	57.3	524-610 bp	1.0	82.1
psbK-psbI_p2	TGGCAAGCAGCTGTAAGTTT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.3	57.3	521-607 bp	1.0	80.1
psbK-psbI_p3	TTGGCAAGCAGCTGTAAGTT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.3	57.3	522-608 bp	1.0	80.1
psbK-psbI_p4	TTTGGCAAGCAGCTGTAAGT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.3	57.3	523-609 bp	1.0	80.1
psbK-psbI_p5	GTTTGGCAAGCAGCTGTAAG	CCGGATTACGTCCTGGATCA	50.0	55.0	57.9	59.0	513-599 bp	1.0	79.7