Hotspot

rps16-trnQ-CAA

LSC · 5995-6466 · 472 bp

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Pi 0.0434

MarkerSeek score 74.70

Primer available yes

Label rps16-trnQ-CAA

Feature Metadata

label_name: rps16-trnQ-CAA
feature_type: igs
region: LSC
length_bp: 472
variable_sites: 28
pi: 0.0434
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-CAA_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	440-545 bp	1.0	86.7
rps16-trnQ-CAA_p2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	444-549 bp	1.0	82.0
rps16-trnQ-CAA_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	470-575 bp	1.0	81.7
rps16-trnQ-CAA_p4	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	432-537 bp	1.0	81.6
rps16-trnQ-CAA_p5	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.9	58.1	439-544 bp	1.0	81.0