Hotspot

trnK-AAA-rps16

LSC · 4236-4876 · 641 bp

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Pi 0.0349

MarkerSeek score 69.30

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 33
pi: 0.0349
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	1002-1047 bp	1.0	84.9
trnK-AAA-rps16_p2	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	55.0	50.0	58.0	58.1	1003-1048 bp	1.0	84.9
trnK-AAA-rps16_p3	CCAAACGGACTGGGGTTAAA	CCCAATGAGCCGTTTATCGA	50.0	50.0	58.0	58.1	1343-1396 bp	1.0	84.4
trnK-AAA-rps16_p4	CCAAACGGACTGGGGTTAAA	TCCCAATGAGCCGTTTATCG	50.0	50.0	58.0	58.1	1344-1397 bp	1.0	84.4
trnK-AAA-rps16_p5	AGCTAGACTGCTAACCGGAT	CCCAATGAGCCGTTTATCGA	50.0	50.0	57.9	58.1	1523-1580 bp	1.0	83.6