Hotspot

ycf1

SSC · 122802-128015 · 5214 bp

Back to Dolomiaea

Pi 0.207

MarkerSeek score 75.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5214
variable_sites: 1804
pi: 0.207
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCAAGCGGCTCCCATTATT	CCTTGGTTTATCCCGAGCAA	50.0	50.0	58.0	57.9	956-1010 bp	0.5	61.4
ycf1_p2	GCGGCTCCCATTATTGGTAT	CCTTGGTTTATCCCGAGCAA	50.0	50.0	57.8	57.9	951-1005 bp	0.5	60.0
ycf1_p3	CTCAAGCGGCTCCCATTATT	TACGGTCTCCTGCTTAACCT	50.0	50.0	58.0	57.8	623 bp	0.5	58.5
ycf1_p4	CTCAAGCGGCTCCCATTATT	AACCTTGGTTTATCCCGAGC	50.0	50.0	58.0	57.9	958-982 bp	0.333333	54.4
ycf1_p5	GCGGCTCCCATTATTGGTAT	AACCTTGGTTTATCCCGAGC	50.0	50.0	57.8	57.9	953-977 bp	0.333333	53.1