Hotspot

ndhI

SSC · 118103-118603 · 501 bp

Back to Dolomiaea

Pi 0.265

MarkerSeek score 67.50

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 221
pi: 0.265
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GCCGTACCATGACGTATCTG	AGCCGAGTGTGAACGATTAC	55.0	50.0	58.2	58.0	1708-1709 bp	0.5	58.3
ndhI_p2	TCACCAAGCAAAAACCCTCA	AGCCGAGTGTGAACGATTAC	45.0	50.0	57.8	58.0	1062 bp	0.333333	52.1
ndhI_p3	TCACCAAGCAAAAACCCTCA	TTTGTGACCTTTGAGGAGCC	45.0	50.0	57.8	58.0	1268 bp	0.333333	52.0
ndhI_p4	TCACCAAGCAAAAACCCTCA	GATGGACTTTACCGAGGCTG	45.0	55.0	57.8	58.1	757 bp	0.333333	51.8
ndhI_p5	TCACCAAGCAAAAACCCTCA	GACCTTTGAGGAGCCGTATG	45.0	55.0	57.8	58.1	1263 bp	0.333333	51.7