Hotspot

ndhH

SSC · 120836-122017 · 1182 bp

Back to Dolomiaea

Pi 0.239

MarkerSeek score 80.00

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 471
pi: 0.239
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CGTTGTTGTATACCCGCAGA	TCAACGGTTGCTGGCTTATT	50.0	45.0	58.0	58.1	1550 bp	0.5	59.4
ndhH_p2	CGTTGTTGTATACCCGCAGA	CAGATGGACATATGCGCTGT	50.0	50.0	58.0	58.1	2045-2061 bp	0.5	59.3
ndhH_p3	TGCGTATTCAGGGCCAATAC	TCAACGGTTGCTGGCTTATT	50.0	45.0	58.0	58.1	1569 bp	0.5	59.1
ndhH_p4	CCTGACATAAGAAGGCCGAC	TCAACGGTTGCTGGCTTATT	55.0	45.0	58.1	58.1	1814 bp	0.5	58.9
ndhH_p5	GACATAAGAAGGCCGACAGG	TCAACGGTTGCTGGCTTATT	55.0	45.0	58.1	58.1	1811 bp	0.5	58.9