Hotspot

ndhG

SSC · 117232-117762 · 531 bp

Back to Dolomiaea

Pi 0.197

MarkerSeek score 75.90

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 175
pi: 0.197
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CGATGAGTTCCCCGAGTTTT	TTGATCACATCCGAGCGTTT	50.0	45.0	57.9	57.9	1908-1911 bp	0.5	58.8
ndhG_p2	GAATCGATGAGTTCCCCGAG	TTGATCACATCCGAGCGTTT	55.0	45.0	57.9	57.9	1912-1915 bp	0.5	58.5
ndhG_p3	CGATGAGTTCCCCGAGTTTT	TCAAATTGCTTTGGGTCGGT	50.0	45.0	57.9	58.3	1633-1636 bp	0.5	57.2
ndhG_p4	CGATGAGTTCCCCGAGTTTT	TGAGGGTTTTTGCTTGGTGA	50.0	45.0	57.9	57.8	1509 bp	0.333333	51.5
ndhG_p5	GAATCGATGAGTTCCCCGAG	TGAGGGTTTTTGCTTGGTGA	55.0	45.0	57.9	57.8	1513 bp	0.333333	51.1