Hotspot

rps15-ycf1

SSC · 122388-122801 · 414 bp

Back to Dolomiaea

Pi 0.258

MarkerSeek score 77.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 414
variable_sites: 179
pi: 0.258
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	AATAATGGGAGCCGCTTGAG	50.0	50.0	58.0	58.0	1044-1060 bp	0.5	60.3
rps15-ycf1_p2	CCGCAGACCTCTCTGAGATA	AATAATGGGAGCCGCTTGAG	55.0	50.0	58.0	58.0	630-646 bp	0.5	60.3
rps15-ycf1_p3	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	45.0	50.0	58.1	58.0	907-923 bp	0.5	59.9
rps15-ycf1_p4	AATAAGCCAGCAACCGTTGA	AATAATGGGAGCCGCTTGAG	45.0	50.0	58.1	58.0	667-683 bp	0.5	59.7
rps15-ycf1_p5	CCAGAATTTTCCGCAGACCT	AATAATGGGAGCCGCTTGAG	50.0	50.0	58.2	58.0	640-656 bp	0.5	59.1