Hotspot

trnL-UAG-ccsA

SSC · 113249-113389 · 141 bp

Back to Dolomiaea

Pi 0.205

MarkerSeek score 67.70

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 141
variable_sites: 50
pi: 0.205
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TCACTCACATTGTGGTGGTG	TGATCCACAAAGAAGAGCCG	50.0	50.0	58.0	57.9	1313-1345 bp	0.5	60.8
trnL-UAG-ccsA_p2	TCTTTCTCGAGAAGGGCAGA	TGATCCACAAAGAAGAGCCG	50.0	50.0	58.1	57.9	1245-1277 bp	0.5	60.4
trnL-UAG-ccsA_p3	GTGAAATCGGTAGACACGCT	TGATCCACAAAGAAGAGCCG	50.0	50.0	58.0	57.9	674-680 bp	0.5	59.5
trnL-UAG-ccsA_p4	TGAAATCGGTAGACACGCTG	TGATCCACAAAGAAGAGCCG	50.0	50.0	58.0	57.9	673-679 bp	0.5	59.5
trnL-UAG-ccsA_p5	TGGTGGTGGGGAGTCTTATT	TGATCCACAAAGAAGAGCCG	50.0	50.0	57.9	57.9	1301-1320 bp	0.333333	53.6