Hotspot

ndhF-rpl32

SSC · 111111-112151 · 1041 bp

Back to Dolomiaea

Pi 0.227

MarkerSeek score 80.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1041
variable_sites: 397
pi: 0.227
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CACCACCACAATGTGAGTGA	50.0	50.0	58.0	58.0	1540-1547 bp	0.5	60.4
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CACCACCACAATGTGAGTGA	45.0	50.0	58.0	58.0	1538-1545 bp	0.5	60.3
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CACCACCACAATGTGAGTGA	45.0	50.0	58.0	58.0	1539-1546 bp	0.5	60.3
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	AATAAGACTCCCCACCACCA	50.0	50.0	58.0	57.9	1552-1559 bp	0.5	60.3
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	AATAAGACTCCCCACCACCA	45.0	50.0	58.0	57.9	1550-1557 bp	0.5	60.2