Hotspot

ndhA

SSC · 114286-116447 · 2162 bp

Back to Dolichoglottis

Pi 0.00325

MarkerSeek score 48.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2162
variable_sites: 7
pi: 0.00325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCATTATGGGAGAAGTTGATCGT	TGTTTGATACAAGTCGTGAA	39.1	35.0	57.4	52.8	1369-2224 bp	1.0	40.3
ndhA_p2	TCATTATGGGAGAAGTTGATCGT	TTGTTTGATACAAGTCGTGA	39.1	35.0	57.4	52.8	1370-2225 bp	1.0	40.3
ndhA_p3	TCATTATGGGAGAAGTTGATCGT	TTGTTTGATACAAGTCGTGAA	39.1	33.3	57.4	53.7	1370-2225 bp	1.0	40.3
ndhA_p4	TCATTATGGGAGAAGTTGATCGT	TGTTTGATACAAGTCGTGAAT	39.1	33.3	57.4	53.5	1369-2224 bp	1.0	40.3
ndhA_p5	TGGGAGAAGTTGATCGTTAA	TGTTTGATACAAGTCGTGAA	40.0	35.0	54.0	52.8	1366-2218 bp	1.0	40.3