Hotspot

ndhA-ndhI

SSC · 116448-116525 · 78 bp

Back to Dolichoglottis

Pi 0.0256

MarkerSeek score 67.20

Primer available yes

Label ndhA-ndhI

Feature Metadata

label_name: ndhA-ndhI
feature_type: igs
region: SSC
length_bp: 78
variable_sites: 2
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhI_p1	GATGGACTTTACCGAGGCTG	TGACCAATATACCTTGCGGC	55.0	50.0	58.1	58.0	268-1369 bp	1.0	79.4
ndhA-ndhI_p2	AGATGGACTTTACCGAGGCT	TGACCAATATACCTTGCGGC	50.0	50.0	58.1	58.0	269-1369 bp	1.0	78.8
ndhA-ndhI_p3	TTACCGAGGCTGAGAATGGA	TGACCAATATACCTTGCGGC	50.0	50.0	58.1	58.0	260-1371 bp	1.0	78.8
ndhA-ndhI_p4	GATGGACTTTACCGAGGCTG	CAATATACCTTGCGGCTCGT	55.0	50.0	58.1	58.1	264-1369 bp	1.0	78.7
ndhA-ndhI_p5	AGATGGACTTTACCGAGGCT	CAATATACCTTGCGGCTCGT	50.0	50.0	58.1	58.1	265-1369 bp	1.0	78.1