Hotspot

atpB-rbcL

LSC · 54103-54840 · 738 bp

Back to Dolichoglottis

Pi 0.00272

MarkerSeek score 60.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 2
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGCCCCAGGTTTGTTTT	GCTTTGAATCCAACACTCGC	45.0	50.0	58.0	58.0	850-1371 bp	1.0	79.9
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	GCTTTGAATCCAACACTCGC	50.0	50.0	58.0	58.0	894-1368 bp	1.0	79.7
atpB-rbcL_p3	CGTGGTAACCGCAGAACTAG	GCTTTGAATCCAACACTCGC	55.0	50.0	58.4	58.0	824-1369 bp	1.0	76.9
atpB-rbcL_p4	GATACGCCCCAGGTTTGTTT	GCTTTGAATCCAACACTCGC	50.0	50.0	58.5	58.0	851-1369 bp	1.0	76.2
atpB-rbcL_p5	CATTTTGCCTGGCGGAAAAG	GCTTTGAATCCAACACTCGC	50.0	50.0	58.6	58.0	902-1370 bp	1.0	75.3