Hotspot

ndhK-ndhC

LSC · 49930-49980 · 51 bp

Back to Dolichoglottis

Pi 0.0196

MarkerSeek score 63.80

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 51
variable_sites: 1
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	ACCATAGAGAAGCGGCCATA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.3	57.8	291-1369 bp	1.0	76.2
ndhK-ndhC_p2	ACCATAGAGAAGCGGCCATA	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.3	57.8	210-1369 bp	1.0	76.2
ndhK-ndhC_p3	ACCATAGAGAAGCGGCCATA	GGGGCATTGGAATGGTCTTA	50.0	50.0	58.3	57.5	192-1368 bp	1.0	74.2
ndhK-ndhC_p4	CCATAGAGAAGCGGCCATAA	TGGGCAATGAGTTTCGATGT	50.0	45.0	57.2	57.8	290-1368 bp	1.0	71.8
ndhK-ndhC_p5	CCATAGAGAAGCGGCCATAA	GTTTATGCATGGCGAAAGGG	50.0	50.0	57.2	57.8	209-1368 bp	1.0	71.7