Hotspot

pafI-trnS-GGA

LSC · 44094-44971 · 878 bp

Back to Dolichoglottis

Pi 0.00346
MarkerSeek score 60.40
Primer available yes
Label pafI-trnS-GGA

Feature Metadata

label_name
pafI-trnS-GGA
feature_type
igs
region
LSC
length_bp
878
variable_sites
3
pi
0.00346
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA ATGCTACGCTTTCAACCACT 45.0 45.0 56.4 57.8 1007-1008 bp 1.0 66.1
pafI-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA ATGCTACGCTTTCAACCACT 45.0 45.0 56.4 57.8 1008-1009 bp 1.0 66.1
pafI-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1050-1051 bp 1.0 65.8
pafI-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1051-1052 bp 1.0 65.8
pafI-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA CCAATGCTACGCTTTCAACC 45.0 50.0 56.4 57.7 1010-1011 bp 1.0 65.5