Hotspot

psaA-pafI

LSC · 41451-42148 · 698 bp

Back to Dolichoglottis

Pi 0.00434

MarkerSeek score 61.10

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 3
pi: 0.00434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	887-890 bp	1.0	76.5
psaA-pafI_p2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	889-892 bp	1.0	76.4
psaA-pafI_p3	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	55.0	45.0	57.8	57.5	888-891 bp	1.0	74.6
psaA-pafI_p4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.5	58.3	822-825 bp	1.0	74.4
psaA-pafI_p5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.5	58.3	824-827 bp	1.0	74.4