Hotspot

trnT-GGU-psbD

LSC · 31255-32443 · 1189 bp

Back to Dolichoglottis

Pi 0.00427

MarkerSeek score 49.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1189
variable_sites: 5
pi: 0.00427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCTTCAAATCCGACAAGGGA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.2	58.0	1295-2362 bp	1.0	79.3
trnT-GGU-psbD_p2	GCTTCAAATCCGACAAGGGA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.2	58.1	1313-2380 bp	1.0	78.4
trnT-GGU-psbD_p3	GCTTCAAATCCGACAAGGGA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.2	58.2	1303-2370 bp	1.0	77.3
trnT-GGU-psbD_p4	GCTTCAAATCCGACAAGGGA	CGAAACGGTCCCTACGTAAC	50.0	55.0	58.2	58.4	1296-2363 bp	1.0	76.3
trnT-GGU-psbD_p5	TCGCTTCAAATCCGACAAGG	GAAACGGTCCCTACGTAACC	50.0	55.0	58.6	58.0	1297-2362 bp	1.0	76.2